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KMID : 0375319920140020269
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Journal of Clinical Pathology and Quality Control
1992 Volume.14 No. 2 p.269 ~ p.285
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A Study on the Effectiveness of disinfectants on Bacteria in Vitro for Contaminated Blood
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Rho Sang-Ok
Kim Yong-Ho
Lee Jeong-Nyeo
Cho Chong-Rae
Hahm Kun-Ju
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Abstract
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Contaminated blood with bacteria is put into the environment without any discontaminated treatment. It may give rise to a serious health and sanitation problems and also to a threat of spreading infectious disease.
In order to select the appropriate disinfectant and disinfection procedure, the tests performed in vitro via artificially contaminated blood with bacteria.
We studied the effectiveness of disinfectants on bacterias and C. parapsilosis using several disinfectants commonly used in hospitals.
The tested disinfectants were sodium hypochlorite(NaOC1), sodium dichloroisocyanurate (NaDCC), ethyl alcohol(EtOH), povidon iodine, cresol soap, benzalkonium chloride, monolauryl-di(aminoethyl)-glycin HC1(MLDGH) and glutaraldehyde.
The bactericidal effects were studied through the dilution and spread plate method. The results were as follows;
1. The effectiveness of disinfectants on the non-spore forming bacteria and C. parapsilosis of the 0.04 % NaOCI and 0.01 % NaDCC was weak and the other chemicals have the strong effectiveness when used according to the manufacturer´s instruction.
2. The effectiveness of disinfectants on the spore forming bacteria of the 1 % glutaraldehyde and 0.25 % NaOC1 was 100 % within 30 minutes and 1 % benzalkonium chloride and 10
povidon iodine was 100 % within 4 hours. 2 % MLDGH was 98.5 % within 5 hours.
In conclusion, this study provided useful information for the 2% benzalkonium chloride is the best disinfectants in consideration of the chemical stability, economy, and sanitation
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KEYWORD
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Chemical disinfections, Bacteria, Spore
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